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ODN 2006 (ODN 7909)

更新時間:2022-01-28

簡要描述:

CpG oligonucleotides (ODNs) are synthetic ODNs that contain unmethylated CpG dinucleotides in specific sequence contexts (CpG motifs).These CpG motifs are present at a 20-fold greater frequency in ……

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Class B CpG oligonucleotide - Human TLR9 ligand

CpG oligonucleotides (ODNs) are synthetic ODNs that contain unmethylated CpG dinucleotides in specific sequence contexts (CpG motifs).

These CpG motifs are present at a 20-fold greater frequency in bacterial DNA than in mammalian DNA. CpG ODNs activate Toll-like receptor 9 (TLR9), leading to strong immunostimulatory effects.

ODN 2006 (ODN 7909) is a class B CpG ODN and is a ligand of choice for human TLR9. Class B CpG ODNs contain a full phosphorothioate backbone with one or more CpG dinucleotides.

They strongly activate B cells but weakly stimulate IFN-α secretion in pDCs.


Specifications

Synonyms: ODN 7909, PF_3512676

Specificity: human TLR9 agonist

Working concentration: 1-5 µM

Solubility:  5 mg/ml in water

ODN 2006 sequence:
5’-tcgtcgttttgtcgttttgtcgtt-3’ (24 mer)
Note: Bases are phosphorothioate (nuclease resistant).

Quality control:
- TLR9 activity has been tested using HEK-Blue™ TLR9 cells.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.


Contents

ODN2006 is provided lyophilized and is available in three quantities:

tlrl-2006 (formerly tlrl-hodnb):

  • 200 μg (25.96 nmol) lyophilized ODN2006

  • 1.5 ml sterile endotoxin-free water

tlrl-2006-1 (formerly tlrl-hodnb-1):

  • 1 mg (129.8 nmol) lyophilized ODN2006

  • 1.5 ml sterile endotoxin-free water

tlrl-2006-5 (formerly tlrl-hodnb-5):

  • 5 mg (649 nmol) lyophilized ODN2006

  • 10 ml sterile endotoxin-free water

 ODN 2006 (ODN 7909) is shipped at room temperature.

 Upon receipt, store at -20?°C.


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